![]() MTT assay was used to measure the percentage of cell viability ( 17). Cell counting was performed using a hemocytometer (HGB, Germany). The culture medium was replaced with fresh medium three times a week. ![]() The cells were incubated in a CO 2 incubator (MEMMERT, IN55) at 37☌ with 5% CO 2 and humidity of 80%. The cells were cultured in 25 mL plastic flasks containing DMEM (DENAzist Asia, Iran), FBS 10% (DENAzist Asia, Iran), and 10µg/mL penicillin-streptomycin solution. The A375 cancer cells were purchased from the Cell Bank of the Iranian Biological Resource Centre, Tehran, Iran. This research was conducted in the Cellular and Molecular Laboratory of Islamic Azad University, Tehran branch (ID: 101290532353935) in 2020. The aim of this study was to investigate the effect of ciprofloxacin and LPL on apoptosis, cell viability, and reactive oxygen species (ROS) Levels in the A375 cell lines. Low-power laser (LPL) can be an alternative treatment option that minimizes the side effects of current treatments used to treat NMSC ( 16). By careful selection of parameters, lasers can be used to target tumor components. Since the advent of the first lasers, their use in medicine has expanded dramatically ( 15). Ciprofloxacin is the most common fluoroquinolone and is more associated with skin side effects than other fluoroquinolones ( 8). These drugs can form cyclobutane thymine dimers (TT CPDs) to break DNA strands and the oxidized bases by both type I and II reactions ( 13, 14). They are also ultraviolet (UV) chromophores with absorption peaks at 280 - 315 nm (UVB) and 315 - 400 nm (UVA). Fluoroquinolones inhibit DNA gyrase and topoisomerase IV enzymes, which are essential for bacterial proliferation ( 12). Fluoroquinolone is structurally based on nalidixic acid, which has been replaced by fluorine C6. They have photo-carcinogenic effects in mice ( 10) and increase the risk of pre-malignant skin lesions in patients ( 11). Fluoroquinolone antibiotics have been identified as a light sensitizer with a wide range of adverse skin reactions ( 9). Light-sensitive activated oxygen species can damage nucleic acids and their precursors and the membrane’s proteins and lipids ( 8). Optical sensitivity can be achieved through an exciting chromophore (optical sensitivity type I) and/or the formation of single oxygen (type II) ( 7). This is due to the ability of drugs or their metabolites to absorb energy from sunlight ( 6) and can significantly increase the risk of non-melanoma skin cancer (NMSC). Sensitivity of the skin to light is one of the side effects of some drugs. According to studies in the Netherlands and the United Kingdom, this could be due to increased daily outdoor activities without adequate clothing, increased seaside travel, prolonged sun exposure, and ozone depletion ( 5). The incidence of skin cancers has increased in recent years ( 4). The origin of skin tumors can be basal layer cells, squamous cells, melanocyte cells (malignant melanoma), immune cells (lymphoma, etc.), skin appendages, etc. Studies have shown that skin cancer is the most common type of cancer in the Middle East ( 2). Skin cancer is one of the most common cancers and affects men more than women ( 1). Synergistic Ciprofloxacin Low Power Laser MTT Assay 1. Because the combination of ciprofloxacin and LPL therapy can stop the proliferation of cancer cells, especially skin cancer cells thus, the use of this treatment can be used to treat cancer in the future.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |